Functional Proteomics Laboratory (FPL)
A*STAR

FUNCTIONAL PROTEOMICS LABORATORY (FPL)

The Functional Proteomics Laboratory at IMCB is a high profile group with a unique portfolio. It gathers broad expertise from diverse fields including biochemistry, immunology, cell biology and mass spectrometry. Our lab’s long-term research interests are focused on the integration of cell biology and immunology with quantitative mass spectrometry-based proteomics. Our expertise includes multi-omic approaches in precision medicine for personalised therapies, antigen identification pipelines development, body fluid biomarker discovery, clinical proteomics and drug target deconvolution.

Expertise

The Functional Proteomics Laboratory team has unique expertise in drug discovery target deconvolution, protein/tissue/fluid biomarker identifications and cell biology bioID. The lab’s advanced mass spectrometry services include antigen identification, antibody specificity and characterization, and targeted proteomics validation (MRM, PRM).

Core Expertise

Biomarker discovery (membrane proteome analysis, secretome analysis, etc.)
Large scale protein identification experiments (ligand identification)
Quantitative proteomics (SILAC, TMT, iTRAQ, di-Methyl)
Quantitative identification of interacting proteins (protein complexes analysis)
Post-translational modification analysis
Signaling networks analysis
DNA-, RNA- protein interactions
Lipids-protein interactions
Recombinant proteins/antibodies quality check
MHC presented peptides identification
Targeted proteomics PRMs (parallel reaction monitoring)

Radoslaw SOBOTA

Co PI & Principal Investigator, FPL

Expertise

Post-translational modifications (PTM), chemical proteomics, non-tryptic peptides, mass spectrometry, biochemistry, clinical proteomics, large scale proteomics studies, industry alignments, precision medicine, targeted pharmacology, targeted therapies, drug-discovery