PROTEIN AND PROTEOMICS CENTRE (PPC)
NUS

PROTEIN AND PROTEOMICS CENTRE (PPC)
A centre for research on proteins, peptides and functional genomics

The Protein and Proteomics Centre (PPC) is a core facility focused on advanced research in protein analytics and mass spectrometry services. Our areas of expertise include biomarker discovery, proteomics, quantitative proteomics, and structural mass spectrometry (Amide Hydrogen/Deuterium Exchange MS).

PPC works closely with both academic and industrial partners in all areas of biological research.

Services & Expertise

At the Protein and Proteomics Centre, our goal is to provide state-of-the-art mass spectrometry services and expertise while expanding MS applications across the Life Sciences spanning biology, chemistry and pharmacy.

We aim to build on our status as a Waters World Centre of Innovation in HDXMS and a SCIEX Regional Centre of Distinction to other areas of mass spectrometry applications through partnerships with individual researchers and industrial collaborations.

Core Services

In-gel Protein Identification
Identification of proteins from gel bands/spots
Shotgun Protein Identification
Identification of proteins from complex protein mixture (e.g. whole cell lysates, co-immunoprecipitation elutes)
Molecular Weight Determination
Determining molecular weight of intact proteins in solution
Post-Translational Modifications
Identify post-translational modifications (e.g. phosphorylation, ubiquitination, sumoylation etc.)

Collaborative Services

Phosphoproteomics
Proteome phosphorylation identification and analysis
Protein Structure, Dynamics and Interactions
Amide hydrogen/deuterium exchange mass spectrometry (HDXMS)
Quantitative Proteomics
Unbiased comparative quantification of proteomes using label-based (TMT) or label-free (DIA/SWATH) technologies
Targeted Mass Spectrometry
Selective relative/absolute quantification of target molecules using multiple reaction monitoring (MRM)

Qingsong LIN

Lead PI & Director, PPC

Expertise

Protein biochemistry; Proteomics and mass spectrometry; Disease biomarker discovery; Chemical proteomics and drug target identification; Aquaporin biomimetic membrane for water desalination and reuse